Early Molecular Immune Responses of Turbot (Scophthalmus maximus L.) following Infection with Aeromonas salmonicida subsp. salmonicida

Turbot aquaculture production is an important economic activity in several countries around the world; nonetheless, incidence of diseases, such furunculosis, caused by etiological agent A. salmonicida subsp. salmonicida, responsible for losses to this industry worldwide. Given perspective, study aimed evaluate early immune responses turbot (S. maximus L.) following infection with salmonicida. For this, 72 fish were individually weighed and randomly distributed into 6 tanks a circulating seawater system. bacterial challenge, half individuals (3 36 individuals) infected using peritoneal injection suspension, while other injected PBS kept as control group. Several factors linked innate response studied, including not only haematological (white blood cells, red haematocrit, haemoglobin, mean corpuscular volume, cell haemoglobin concentration, neutrophils, monocytes, lymphocytes, thrombocytes) oxidative stress parameters, but also analyses expression 13 key immune-related genes (tnf-α, il-1β, il-8, pparα-1, acox1, tgf-β1, nf-kB p65, srebp-1, il-10, c3, cpt1a, pcna, il-22). No significant differences recorded or humoral parameters (lysozyme, anti-protease, peroxidase) at selected sampling points. There was neither any evidence changes levels indicators (catalase, glutathione S-transferase, lipid peroxidation, superoxide dismutase). In contrast, pro-inflammatory il-1β), anti-inflammatory (il-10), (c3) up-regulated, another gene metabolism (acox1) down-regulated. The results showed new insights about